In this study, we explore a steroid compound from the endophytic of the mangrove (Avicennia marina). Penicillium sp. is one of the microbes endophytic isolated from the mangrove and induced in the chitin medium by the so-called "cultivation". The cultivation in the medium is a strategy to enhance secondary metabolites production. In addition, the steroid compound of the secondary metabolites can be extracted and isolated from the purified fraction, followed by analysis and characterization to validate the target compound. Using the acquired 14MA15 fraction as a representative sample, we found that it presents a peak in the UV spectrum at a wavelength of 206 nm from the carbonyl group. The stretching vibration of C=O group in the IR spectrum is also confirmed at 1712 cm-1. Furthermore, other stretching vibrations from the O-H group, C-O group, and –C–H group are detected at 3410 cm-1, 1055 cm-1, and 1463 - 723 cm-1, respectively. Therefore, it is concluded that the steroid compound proposed has a molecular formula of C26H40O2 according to MS fragmentation analysis.